Research Program

Current Projects

  • Microbiome
  • E. coli evolution
  • Cancer metabolism
  • CHO cell culture
  • Sustainable biofuels
  • Algae, cyanobacteria

Research Interests

Antoniewicz Laboratory

Metabolic engineering, biotechnology, cancer, metabolism, microbial communities.

Antoniewicz Lab is developing next generation tools and techniques for analyzing, engineering and manipulating microbial and mammalian cells applied to specific problems in biotechnology (biofuels, pharmaceuticals) and medicine (cancer, obesity, diabetes). In our research we make use of modern techniques for cell culture, 13C metabolic flux analysis, mass spectrometry, molecular biology, bioinformatics and computational biology. Current interests include: elucidating syntrophic interactions in microbial communities, analysis of compartment-specific fluxes in mammalian cells, dynamic flux analysis, cancer metabolism, and engineering microbes for enhanced utilization of renewable substrates and production of value-added chemicals.


Current Projects

Microbiome Microbial communities and microbiome
We are elucidating interactions in synthetic and natural microbial communities using novel 13C-metabolic flux analysis techniques develped in our lab.

E. coli metabolism E. coli metabolism and adaptive evolution
We are studying E. coli metabolism by quantifying cellular responses to genetic perturbations and measuring flux rewiring after adaptive laboratory evolution.

Cancer metabolism Cancer metabolism
We are elucidating altered metabolism of cancer cells and identifying novel therapeutic targets to combat cancer.

CHO cells CHO cell culture
We are studying dynamic metabolism of Chinese hamster ovary (CHO) cells in fed-batch culture and developing new strategies to improve protein production.

Metabolic Engineering Metabolic engineering for sustainability
We are engineering microbial systems for the biological production of renewable chemicals and fuels from carbohydrates and gasses (CO2, syngas).

Algae Algae and cyanobacteria
We are studying metabolism of microalgae and cyanobacteria and identifying new metabolic engineering targets to improve photosynthetic CO2 fixation.


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RESEARCH AREAS

Metabolic Engineering

Metabolic Engineering

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Mammalian Cell Culture

Mammalian Cell Culture

Metabolic Flux Analysis

Metabolic Flux Analysis