WEN GROUP
PROTEIN-ASSEMBLY ENGINEERING LAB



Journal Articles


  1. Luke Francesco Bugada, Mason R. Smith, and Fei Wen. Engineering Spatially Organized Multi-enzyme Assemblies for Complex Chemical Transformation. ACS Catal., 2018, 8: 7898-7906. Download PDF

  2. Christine Yee, Andrew Joseph Zak, Brett Dallas Hill, Fei Wen. The Coming Age of Insect Cells for Manufacturing and Development of Protein Therapeutics. Ind. Eng. Chem. Res, 2018, 57 (31): 10061-10070. Download PDF

  3. Mason R. Smith, Stephanie V. Tolbert, Fei Wen. Protein-scaffold Directed Nanoscale Assembly of T Cell Ligands: Artificial Antigen Presentation with Defined Valency, Density and Ratio. ACS Synthetic Biology, 2018, 7 (6): 1629-1639. Download PDF

  4. Brett D. Hill, Andrew Zak, Eshita Khera, Fei Wen. Engineering Virus-like Particles for Antigen and Drug Delivery, Current Protein & Peptide Science, 2018, 19: 112-127. Download PDF

  5. Ming Huang, Wenjun Huang, Fei Wen, and Ronald G. Larson. Estimation of Binding Free Energies between Peptides and an MHC Class II Molecule Using Course-Grained Molecular Dynamics with a Weighted Histogram Analysis Method. Journal of Computational Chemistry 2017, 38: 2007-2019. Download PDF

  6. Hui Gao, Tae-Su Kim, Primata Mardina, Pengji Zhou, Fei Wen and Jung-Kul Lee. Rare sugar production by coupling of NADH oxidase and L-arabinitol dehydrogenase. RSC Advances, 2016, 6: 66609-66616. Download PDF

  7. Fei Wen and Eshita Khera. “IDENTIFY-SOLVE-BROADCAST YOUR OWN TRANSPORT PHENOMENON: Student-Created YouTube Videos to Foster Active Learning in Mass and Heat Transfer”. Chemical Engineering Education, 2016, Vol. 50, No. 3. Download PDF

  8. Mason Smith, Eshita Khera, Fei Wen. “Engineering Novel and Improved Biocatalyst by Cell Surface Display”. Industrial and Engineering Chemistry Research, 2015, 54 (16): 4021-4032 Download PDF

  9. Hui Gao, In-Won Kim, Joon-Ho Choi, Eshita Khera, Fei Wen*, Jung-Kul Lee*. “Repeated production of L-xylulose by an immobilized whole-cell biocatalyst harboring L-arabinitol dehydrogenase coupled with an NAD+ regeneration system”. Biochemical Engineering Journal. 2015, 96: 23-28, *Denotes corresponding author Download PDF

  10. Hui Gao, Eshita Khera, Jung-Kul Lee*,Fei Wen*. “Immobilization of multi-biocatalysts in alginate beads for cofactor regeneration and improved reusability”. Journal of Visual Experiments, 2016 (PMID:27166648) *Denotes corresponding author. Download PDF

  11. Fei Wen, Huimin Zhao. Construction and Screening of An Antigen-derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification. In Methods in Molecular Biology. 2013, 1061: 245-64 (PMID: 23963942) Download PDF

  12. Jie Sun, Fei Wen, Tong Si, Jianhe Xu, Huimin Zhao. Direct Conversion of Xylan to Ethanol by Recombinant Saccharomyces cerevisiae Strains Displaying an Engineered Mini-hemicellulosome. Applied and Environmental Microbiology, 2012, 78(11): 3837-45. (PMID: 22447594) Download PDF

  13. Jie Sun, Zengyi Shao, Hua Zhao, Nikhil Nair, Fei Wen, Jianhe Xu, Huimin Zhao. Cloning and Characterization of a Panel of Constitutive Promoters for Applications in Pathway Engineering in Saccharomyces cerevisiae. Biotechnology and Bioengineering, 2012, 109(8): 2082-92. (PMID: 22383307)Download PDF

  14. Fei Wen, Dhruv Sethi, Kai Wucherpfennig, Huimin Zhao. Cell surface display of functional human MHC class II proteins: yeast display versus insect cell display. Protein Engineering, Design, and Selection, 2011, 24(9): 701-9. (PMID: 21752831) Download PDF

  15. Fei Wen, Jie Sun, Huimin Zhao. Yeast Surface Display of Trifunctional Minicellulosomes for Simultaneous Saccharification and Fermentation of Cellulose to Ethanol. Applied and Environmental Microbiology, 2010, 76(4): 1251-60. (PMID: 20023102) Download PDF

  16. Fei Wen, Nikhil U. Nair, Huimin Zhao. Protein Engineering in Designing Tailored Enzymes and Microorganisms for Biofuels Production. Current Opinion in Biotechnology, 2009, 20 (4): 412-9. (PMID: 19660930) Download PDF

  17. Zhilei Chen*, Fei Wen*, Ning Sun, Huimin Zhao. Directed Evolution of Homing Endonuclease I-SceI with Altered Sequence Specificity. Protein Engineering, Design, and Selection, 2009, 22(4): 249-56. (PMID: 19176595) * Denotes shared 1st authorship.Download PDF

  18. Fei Wen, Olga Esteban, Huimin Zhao. Rapid Identification of CD4+ T-cell Epitopes Using Yeast Displaying Pathogen-derived Peptide Library. Journal of Immunological Methods, 2008, 336(1): 37-44. (PMID: 18448115) Download PDF

  19. Huimin Yu, Yue Shi, Zhuoling Tian, Fei Wen, Zhongyao, Shen. Heterogenous Expression of Nitrile Hydratase Gene of Nocardia sp. in Recombinant Pichia pastoris. Acta Microbiologica Sinica. 2005, 45 (6): 959-962. (PMID: 16496712) Download PDF



Book Chapters


  1. Fei Wen, Sheryl Rubin-Pitel, Huimin Zhao. Engineering of Therapeutic Proteins. In Protein Engineering and Design, Sheldon J. Park and Jennifer R. Cochran., CRC Press, Taylor & Francis Group, FL. 2009, pp. 153-177. Download PDF

  2. Fei Wen, Michael McLachlan, Huimin Zhao. Directed Evolution, Novel and Improved Enzymes. In Wiley Encyclopedia of Chemical Biology, Tadhg P. Begley, John Wiley & Sons, Inc. Hoboken, NJ. 2008, doi:10.1002/9780470048672.wecb125. Download PDF

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