3D visualization of high-dimensional single-cell datasets
Our laboratory aims to engineer the function of protein assemblies, consisting of an array of complexed or non-complexed proteins, to solve problems in human health and sustainable energy.
Protein engineering, a relatively young field, has played a pivotal role in bringing biotechnology to the forefront of academic research and industrial practice. By focusing on the engineering of single target proteins, a great number of value-added natural and unnatural proteins have been successfully generated and commercialized, such as monoclonal antibodies, industrial enzymes, protein pharmaceuticals, etc. Looking forward to solve problems in more complex biological systems, our laboratory aims to engineer protein assemblies consisting of multiple proteins as a single entity to act in an orchestrated and synergistic manner to carry out specific and more sophisticated biological functions, such as immune-modulation and cascade catalysis. To meet our goals, the protein-assembly engineering must be in sync with an in-depth understanding of the biology underpinning individual problems. As such, our research program puts a balanced emphasis on both technology development and fundamental science. The key to our approach is thus highly interdisciplinary and involves state-of-the-art biology, chemistry, immunology, and biostatistics, with protein engineering principles (directed evolution and rational design) serving as the foundation.
Xiao Yin, Bryan, and Prabhu's paper titled "Segmentation and evaluation of pathway module efficiency: Quantitative approach to monitor and overcome evolving bottlenecks in xylose to ethanol pathway" has been published in Bioresource Technology. Read more
Prof. Wen, Luke and Christine presented their work at the AIChE Annual Meeting held in Orlando, FL.
Xiao Yin has successfully defended her thesis entitled "Quantitative Methods to Engineer Yeast Whole-Cell Biocatalysts for Lignocellulosic Biomass Transformation to Biofuels". Congratulations, Dr. Ma!
Andrew, Trang, Christine, Monem and Prabhu's paper titled "Pseudotyping Improves the Yield of Functional SARS-CoV-2 Virus-like Particles (VLPs) as Tools for Vaccine and Therapeutic Development" has been published in IJMS. Read more
Andrew has successfully defended his thesis entitled "Improving Yield and Quality of Virus-like Particle Vaccines for Respiratory Viruses with Pandemic Potential". Congratulations, Dr. Zak!
Bryan has successfully passed his prelim exam. Congratulations!
Nhi has successfully passed her Dissertation Candidacy Exam and become a PhD candidate. Congratulations, Nhi!
Trang presented “Affordable universal influenza vaccine: M2-based virus-like particles (VLPs)” at the ACS National Meeting held in Indianapolis, IN.
Xiao Yin presented “Engineering Compositionally Uniform Cellulolytic Yeast Whole Cell Biocatalysts with Maximized Enzyme Density via CRISPR/Cas9-Mediated Integration” at the 5th International Conference on CRISPR Technologies held in Berkeley, CA.
Nhi Nguyen joined the Wen Lab as a new PhD student. Welcome!
Luke and Mason's book chapter titled "Rapid Identification of MHCII-Binding Peptides Through Microsphere-Assisted Peptide Screening (MAPS)" has been published in T-Cell Repertoire Characterization. This book provides a valuable compilation of experimental and computational approaches to better understand the heterogeneity and functional complexity of T cells. Congratulations! Read more
Trang has successfully passed her prelim exam. Congratulations!
Our collaborative work with Dr. Kahlenberg's group titled "Imaging mass cytometry reveals predominant innate immune signature and endothelial‐immune cell interaction in juvenile myositis compared to lupus skin" has been published in Arthritis & Rheumatology. Read more
Our collaborative work with Dr. Sun's group titled "Albumin nanoparticle containing a PI3Kγ inhibitor and paclitaxel in combination with α-PD1 induces tumor remission of breast cancer in mice" has been published in Science Translational Medicine. Luke designed and performed the CyTOF experiment and conducted the data analysis. Read more
Bryan has successfully passed his Dissertation Candidacy Exam and become a PhD candidate. Congratulations, Bryan!
Our collaborative work with Dr. Kahlenberg's group titled "Nonlesional lupus skin contributes to inflammatory education of myeloid cells and primes for cutaneous inflammation" has been published in Science Translational Medicine. Christine and Monem designed and performed the imaging CyTOF work. Read more
Our collaborative work with Dr. Kahlenberg's group titled "B cell signatures distinguish cutaneous lupus erythematosus subtypes and the presence of systemic disease activity" has been published in Frontiers in Immunology. Christine and Monem designed and performed the imaging CyTOF work. Read more
Bryan Coleman joined the Wen Lab as a new PhD student. Welcome!
Xiao Yin, Brett and Trang's paper titled "Virus-inspired Strategies for Cancer Therapy" has been published in Seminars in Cancer Biology. Read more
Andrew, Luke and Xiao Yin's paper titled "Virtual versus In-Person Presentation as a Project Deliverable Differentially Impacts Student Engaged-Learning Outcomes in a Chemical Engineering Core Course" has been published in Journal of Chemical Education.Read more
Our collaborative work with Dr. Zou's group titled "Liver metastasis restrains immunotherapy efficacy via macrophage-mediated T cell elimination" has been published in Nature Medicine. Monem designed and performed the CyTOF experiments. Read more
Xiao Yin and Christine presented their work at the AIChE Virtual Annual Meeting.
Trang Hoang joined the Wen Lab as a new PhD student. Welcome!
Our collaborative work with Dr. Gudjonsson's group titled "Contribution of plasma cells and B cells to hidradenitis suppurativa pathogenesis" has been published in JCI Insight. Christine and Monem designed and performed the CyTOF experiments and analysis. Read more
Xiao Yin has successfully passed her prelim exam. Congratulations!
Brett has successfully defended his thesis entitled "Development of a Yeast Biosynthetic Platform for Rapid Production and Engineering of an Influenza Virus-like Particle Vaccine". Congratulations, Dr. Hill!
Our collaborative work Dr. Kamada's group titled "The Intermucosal Connection between the Mouth and Gut in Commensal Pathobiont-Driven Colitis" has been published in Cell. Brett and Monem performed the CyTOF experiments and analysis. Read more
Brett, Prabhu and Monem's paper titled "Yeast Intracellular Staining (yICS): Enabling High-throughput, Quantitative Detection of Intracellular Proteins via Flow Cytometry for Pathway Engineering" has been published in ACS Synthetic Biology. Read more
Our COVID-19 research and vaccine development has been featured by the Michigan Engineering! Read More
Luke and Andrew won the second place in the professional category of the AIChE k-12 STEM competition. Congratulations! Read more
Our VLP work has been featured by the Michigan Engineering! Read More
Andrew, Brett, Monem, Mason and Maddie's paper titled "Enhancing the Yield and Quality of Influenza Virus-like Particles (VLPs) Produced in Insect Cells by Inhibiting Cytopathic Effects of Matrix Protein M2" has been published in ACS Synthetic Biology.
Prof. Wen has been awarded the Young Alumni Achievement Award by the ChBE Department at the University of Illinois Urbana-Champaign. Read more
Prof. Wen and Mason's work "Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification" has been published in Methods in Molecular Biology.
Our work on whole-cell cooperative enzyme catalysis has been featured by Michigan Engineering! Read more
Mason and Hui's paper "Elucidating structure–performance relationships in whole-cell cooperative enzyme catalysis" was published in Nature Catalysis. Congrats!
Andrew and Luke have been accepted to present their work at the AIChE Annual Meeting this year in Orlando, FL. Congratulations!
Mason and Luke's paper "Rapid Microsphere Assisted Peptide Screening (MAPS) of Promiscuous MHCII‐Binding Peptides in Zika Virus Envelope Protein" has been published in AIChE Journal. Congrats!
Prof. Wen has been promoted to Associate Professor of Chemical Engineering.
Xiao Yin has successfully passed her Dissertation Candidacy Exam and become a PhD candidate. Congratulations, Xiao Yin!
Our collaborative work with Dr. Gudjonsson's group titled "The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity" has been published in JCI Insight. Read more
Prof. Wen was awarded the UM College of Engineering David E. Liddle Research Excellence Award for 2018-2019. Read more
Mason, Brett, Andrew and Christine presented their work at the AIChE Annual Meeting held in Pittsburgh, PA.
Xiao Yin joined the Wen Lab as a new PhD student. Welcome!
Prof Wen was named ACS Industrial & Engineering Chemistry Research's "2018 Class of Influential Researchers" Read more
Mason has successfully defended his thesis entitled "Quantitative Approach to Supramolecular Assembly Engineering for Isolating and Activating Antigen-Specific T Cells". Congratulations, Dr. Smith!
Luke and Mason's paper "Engineering Spatially Organized Multi-enzyme Assemblies for Complex Chemical Transformation" was published in ACS Catalysis.
Christine, Brett and Andrew's paper "The Coming Age of Insect Cells for Manufacturing and Development of Protein Therapeutics" was published in I&EC Research.
Mason's paper on Protein-Scaffold Directed Nanoscale Assembly of T Cell Ligands was published in ACS Synthetic Biology.
Prof. Wen was awarded the NSF CAREER Award. Read more
Brett and Andrew's paper "Engineering Virus-like Particles for Antigen and Drug Delivery" was published in Current Protein & Peptide Science.
Prof. Wen was awarded funding by NSF/BME for immune profiling of T cells towards reproducible and scalable biomanufacturing process in personalized cancer immunotherapy
Prof. Wen's paper reporting her pedagogical research on active student learning was published in Chemical Engineering Education.
Hui's paper "Rare sugar production by coupling of NADH oxidase and L-arabinitol dehydrogenase" was published in RSC Advance.
Hui's paper "Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability" was published in Journal of Visualized Experiments.
Prof. Wen was awarded funding by NIH/ORIP for acquiring CyTOF (cytometry by time-of-flight) for investigating single cell proteomics
Prof. Wen has been awarded CRLT's Investigating Student Learning Grant, 2016, funded by the Center for Research on Learning and Teaching, the Office of the Vice Provost for Global and Engaged Education, and the College of Engineering.
Prof. Wen was awarded funding by NIH/NCI to develop new antigen presentation systems and by NSF/BME to engineer protein assemblies for personalized cancer immunotherapy
Prof. Wen and her ChE342 undergraduate students welcomed 88 high school students in their classroom for their first-time U of M experience.
The Wen Lab has research opportunities for undergraduate and graduate students. Read more
Prof. Wen is developing outreach activities with the aims to foster engagement of K-12 students with UM undergraduate and graduate students.Read more